Expected Zoology viva voce questions - B.Sc Life Science - Ist year

1Plasmid dna isolation

Q1 What is a plasmid?
Q2 What is genomic dna?
Q 3 What is the need of plasmid isolation?
Q4 What is the protocol of plasmid isolation?
Q5 What is the name of protocol used for plasmid isolation?
Q6 What is GTE? What are the function of these chemicals?

Q7 Why genomic DNA doesn't come by using GTE method?
Q8 What is a plasmid vector? Give two examples.
Q9 Which plasmid have you isolated? What is its size?


Q10 What is AGE?
Q11 What is agarose?
Q12 Why the gel electrophoresis is horizontal and not vertical as PAGE?
Q13 What is the principle of Agarose gel electrophoresis?
Q14 What is the optimal voltage for plasmid separation on gel electrophoresis?
Q15 Which buffer is used for gel preparation and as running buffer?
Q16 What is the composition of TBE?

Q17 Which stain is used for DNA staining?
Q18 What is the name of interaction that occurs between DNA and EtBr?
Q19 How does EtBr function?
Q20 Which color does EtBr give to DNA (Hint: It's not a color)?
Q21 What is the difference between fluorescence and phosphorescence?
Q22 Why EtBr is considered dangerous?

Q22 How EtBr is safely disposed off?

2 Meiosis
Q1 Name various stages of meiosis.

Q2 What are the various substages of meiosis?

Q3 Where does meiosis occus in animals?

Q4 Where does meiosis occus in plants?
Q5 At what stage crossing over occurs?
Q6 What happens in zygotene?
Q7 What is the significance of meiosis?
Q8 Which division is equational?
Q9 Which division is reductional?
Q10 In what form the chromosomes are seen in metaphase polar view?
Q11 How do you identify leptotene?
Q12 Name the stages of cell cycle.

3TLC
Q1 Explain the principle of TLC.
Q2 What is rf value?
Q3 If a solute travel less, what does it mean?
Q4 How silica is activated?
Q5 What is paper chromatograpy?
Q6 what is the mobile phase?
Q7 what is the stationary phase?

4 Janus green
Q1 What is the principle of this staining?
Q2 Which cytochrome helps in this reaction?
Q3 Why is it called vital stain?
Q4Why bubble is introduced?
Q5Why the cells are kept alive?

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Good Luck?

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